Technical Details

Animal feed samples are analysed to determine the concentration of 12 of the major mycotoxins:

  • Deoxynivalenol
  • Zearalenone
  • Fumonisin B1 and B2
  • T2 and HT2 Toxin
  • Aflatoxin B1, B2, G1, and G2
  • Ochratoxin A
  • Diacetoxyscirpenol

Our scope of accreditation covers all animal feed materials, including forage, grains, and compound feed.

All analyses are conducted using a purpose developed ultra-high performance liquid chromatography mass spectrometry (UHPLC-MS/MS) method.

Using state-of-the-art technology allows us to accurately measure very low levels of mycotoxins in a single method, saving both time and resources.  Limits of detection for each toxin are as follows:

Mycotoxin Limit of Detection (ppb)
Deoxynivalenol 4
Zearalenone 4
Fumonisin B1 and B2 10
T2 Toxin 4
HT2 Toxin 10
Aflatoxin B1, B2, G1, and G2 1
Ochratoxin A 4
Diacetoxyscirpenol 4


Isotopically Labelled Internal Standards


Although UHPLC-MS/MS analysis is a very powerful analytical technique, it is susceptible to an effect known as matrix interference.  Signals from a mass spectrometer can be supressed (or enhanced) by the sample material, yielding inaccurate results.

Mycocheck uses carbon 13 versions of each mycotoxin as reference standards for every analysis.  Using these standards allows the matrix effect to be corrected and accurate results achieved for each toxin, across the whole range of different feed materials.



The concentration of each mycotoxin is reported in a simple to understand report, corrected to a dry matter content of 88%.  An example certificate of results can be viewed below.

Example Results Certificate
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An example certificate demonstrating the layout of mycotoxin analysis results.

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